Proinsulin processing in electrically permeabilized rat islets of Langerhans.

@article{Slee1990ProinsulinPI,
  title={Proinsulin processing in electrically permeabilized rat islets of Langerhans.},
  author={D J Slee and Peter Martin Jones and Simon James Howell},
  journal={Journal of molecular endocrinology},
  year={1990},
  volume={5 3},
  pages={275-80}
}
Proinsulin conversion to insulin was studied using electrically permeabilized rat islets of Langerhans. Using high-performance liquid chromotography separation of radiolabelled islet proteins, we have demonstrated that conversion was dependent upon temperature, sensitive to pH and regulated by MgATP. Ammonium acetate was used to collapse the granular pH gradient, over a pH range of 3.5-7.4. Conversion was optimum at pH 4.5-5.5 and was reduced, but not abolished, at pH 7.4. Ca2+ (10 microM) and… CONTINUE READING

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