Progression of T cell lineage restriction in the earliest subpopulation of murine adult thymus visualized by the expression of lck proximal promoter activity.

@article{Shimizu2001ProgressionOT,
  title={Progression of T cell lineage restriction in the earliest subpopulation of murine adult thymus visualized by the expression of lck proximal promoter activity.},
  author={Chiori Shimizu and Hiroshi Kawamoto and Masakatsu Yamashita and Motoko Kimura and E. Kondou and Yoshikatsu Kaneko and Seiji Okada and Takeshi Tokuhisa and Minesuke Yokoyama and Masaru Taniguchi and Yoshimoto Katsura and Toshinori Nakayama},
  journal={International immunology},
  year={2001},
  volume={13 1},
  pages={105-17}
}
The proximal promoter of lck directs gene expression exclusively in T cells. To investigate the developmental regulation of the lck proximal promoter activity and its relationship to T cell lineage commitment, a green fluorescence protein (GFP) transgenic (Tg) mouse in which the GFP expression is under the control of the proximal promoter of lck was created. In the adult GFP-Tg mice, >90% of CD4(+)CD8(+) and CD4(+)CD8(-) thymocytes, and the majority of CD4(-)CD8(+) and CD4(-)CD8(-) [double… CONTINUE READING