Progranulin directly binds to the CRD2 and CRD3 of TNFR extracellular domains.

  title={Progranulin directly binds to the CRD2 and CRD3 of TNFR extracellular domains.},
  author={Jinlong Jian and Shuai Zhao and Qingyun Tian and Elena Gonzalez-Gugel and Jyoti Joshi Mundra and Sardar M Z Uddin and Ben Liu and Brendon Richbourgh and Ryan Brunetti and Chuan-Ju Liu},
  journal={FEBS letters},
  volume={587 21},
We previously reported that PGRN directly bound to TNF receptors (TNFR) in vitro and in chondrocytes (Tang, et al., Science, 2011). Here we report that PGRN also associated with TNFR in splenocytes, and inhibited the binding of TNFα to immune cells. Proper folding of PGRN is essential for its binding to TNFR, as DTT treatment abolished its binding to TNFR. In contrast, the binding of PGRN to Sortilin was enhanced by DTT. Protein interaction assays with mutants of the TNFR extracellular domain… CONTINUE READING


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  • Z. A. Miller, K. P. Rankin, +19 authors W. W. Seeley
  • J. Neurol. Neurosurg. Psychiatry
  • 2013
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