Programmable deletion, replacement, integration, and inversion of large DNA sequences with twin prime editing

@article{Anzalone2021ProgrammableDR,
  title={Programmable deletion, replacement, integration, and inversion of large DNA sequences with twin prime editing},
  author={Andrew V. Anzalone and Xin D. Gao and Christopher J. Podracky and Andrew T. Nelson and Luke W Koblan and Aditya Raguram and Jonathan M. Levy and Jaron A. M. Mercer and David R. Liu},
  journal={Nature biotechnology},
  year={2021},
  volume={40},
  pages={731 - 740}
}
The targeted deletion, replacement, integration, or inversion of genomic sequences could be used to study or treat human genetic diseases, but existing methods typically require double-strand DNA breaks (DSBs) that lead to undesired consequences including uncontrolled indel mixtures and chromosomal abnormalities. Here, we describe twin prime editing (twinPE), a DSB-independent method that uses a prime editor (PE) protein and two prime editing guide RNAs (pegRNAs) for the programmable… 

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