Programmable RNA-binding protein composed of repeats of a single modular unit.

  title={Programmable RNA-binding protein composed of repeats of a single modular unit.},
  author={Katarzyna P Adamala and Daniel A Martin-Alarcon and Edward S. Boyden},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={113 19},
The ability to monitor and perturb RNAs in living cells would benefit greatly from a modular protein architecture that targets unmodified RNA sequences in a programmable way. We report that the RNA-binding protein PumHD (Pumilio homology domain), which has been widely used in native and modified form for targeting RNA, can be engineered to yield a set of four canonical protein modules, each of which targets one RNA base. These modules (which we call Pumby, for Pumilio-based assembly) can be… CONTINUE READING
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