Programmable RNA N6-methyladenosine editing by CRISPR-Cas9 conjugates

@article{Liu2019ProgrammableRN,
  title={Programmable RNA N6-methyladenosine editing by CRISPR-Cas9 conjugates},
  author={Xiao-Min Liu and Jun Zhou and Yuanhui Mao and Quanquan Ji and Shu-Bing Qian},
  journal={Nature chemical biology},
  year={2019},
  volume={15},
  pages={865 - 871}
}
RNA modification in the form of N6-methyladenosine (m6A) regulates nearly all the post-transcriptional processes. The asymmetric m6A deposition suggests that regional methylation may have distinct functional consequences. However, current RNA biology tools do not distinguish the contribution of individual m6A modifications. Here we report the development of ‘m6A editing’, a powerful approach that enables m6A installation and erasure from cellular RNAs without changing the primary sequence. We… 
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