Programmable DNA cleavage in vitro by Cas9.

Abstract

The ternary Cas9-crRNA-tracrRNA complex (Cas9t) of the Type II CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated) system functions as an Mg2+-dependent RNA-directed DNA endonuclease that locates its DNA target guided by the crRNA (CRISPR RNA) in the tracrRNA-crRNA structure and introduces a double-strand break at a… (More)
DOI: 10.1042/BST20130164

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