Progesterone secretion and proliferation in cultured rabbit granulosa cells under conditions of beta-D-xyloside-induced inhibition of proteoglycan synthesis.

@article{Benham1995ProgesteroneSA,
  title={Progesterone secretion and proliferation in cultured rabbit granulosa cells under conditions of beta-D-xyloside-induced inhibition of proteoglycan synthesis.},
  author={Annie Benha{\"i}m and Colette F{\'e}ral and Monique Langris and J. C. Bocquet and Pierre Leymarie},
  journal={Biology of reproduction},
  year={1995},
  volume={52 4},
  pages={
          939-46
        }
}
Proteoglycans present in follicular fluid are synthesized by granulosa cells under gonadotropin control. An inhibitor of proteoglycan synthesis, p-nitrophenyl-beta-D-xyloside (beta-D-xyloside) was used as a probe to study rabbit granulosa cell steroidogenesis and proliferation under abrogated proteoglycan synthesis. Granulosa cells isolated from rabbit preovulatory follicles were cultured 24 h in Minimum Essential Medium plus 2.5% fetal calf serum in the presence or absence of beta-D-xyloside… CONTINUE READING
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