Profiling the immune response in patients with breast cancer by phage-displayed cDNA libraries.

@article{Sioud2001ProfilingTI,
  title={Profiling the immune response in patients with breast cancer by phage-displayed cDNA libraries.},
  author={Mouldy Sioud and Morris H. Hansen},
  journal={European journal of immunology},
  year={2001},
  volume={31 3},
  pages={716-25}
}
Display on the surface of filamentous phages has been shown to be well suited for the enrichment of serum antibody-binding ligands. Here, we have taken the advantage of this technology to analyze the humoral immune response in patients with cancer. The cDNA repertoires from breast cancer cell lines T47D and MCF-7 were fused to the 3'-end of the filamentous phage M13 gene VI in all three reading frames. When the libraries were biopanned on rabbit polyclonal IgG against the human Bcl-x(L) protein… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 23 extracted citations

Similar Papers

Loading similar papers…