Profiling the HeLa S3 transcriptome using randomly primed cDNA and massively parallel short-read sequencing.

@article{Morin2008ProfilingTH,
  title={Profiling the HeLa S3 transcriptome using randomly primed cDNA and massively parallel short-read sequencing.},
  author={Ryan D. Morin and Matthew N. Bainbridge and Anthony P. Fejes and Martin Hirst and Martin Krzywinski and Trevor J. Pugh and Helen McDonald and Richard Varhol and Steven Jones and Marco A. Marra},
  journal={BioTechniques},
  year={2008},
  volume={45 1},
  pages={81-94}
}
Sequence-based methods for transcriptome characterization have typically relied on generation of either serial analysis of gene expression tags or expressed sequence tags. Although such approaches have the potential to enumerate transcripts by counting sequence tags derived from them, they typically do not robustly survey the majority of transcripts along their entire length. Here we show that massively parallel sequencing of randomly primed cDNAs, using a next-generation sequencing-by… CONTINUE READING
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