Profiling of ribose methylations in RNA by high-throughput sequencing.

@article{Birkedal2015ProfilingOR,
  title={Profiling of ribose methylations in RNA by high-throughput sequencing.},
  author={Ulf Birkedal and Mikkel Christensen-Dalsgaard and Nicolai Krogh and Radhakrishnan Sabarinathan and Jan Gorodkin and Henrik Nielsen},
  journal={Angewandte Chemie},
  year={2015},
  volume={54 2},
  pages={
          451-5
        }
}
Ribose methylations are the most abundant chemical modifications of ribosomal RNA and are critical for ribosome assembly and fidelity of translation. Many aspects of ribose methylations have been difficult to study due to lack of efficient mapping methods. Here, we present a sequencing-based method (RiboMeth-seq) and its application to yeast ribosomes, presently the best-studied eukaryotic model system. We demonstrate detection of the known as well as new modifications, reveal partial… CONTINUE READING
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