Profiling of membrane protein variants in a baculovirus system by coupling cell-surface detection with small-scale parallel expression.

@article{Hanson2007ProfilingOM,
  title={Profiling of membrane protein variants in a baculovirus system by coupling cell-surface detection with small-scale parallel expression.},
  author={Michael A. Hanson and Alexei Brooun and Kent A. Baker and Veli-Pekka Jaakola and Chris Z. Roth and Ellen Yu-Lin Chien and Alexander B. Alexandrov and Jeffrey Velasquez and Leila K Davis and M. M. Griffith and K. Ang Chai Moy and Barbie K Ganser-Pornillos and Yuanzi Hua and Peter A Kuhn and Sam Ellis and Mark Yeager and Raymond C. Stevens},
  journal={Protein expression and purification},
  year={2007},
  volume={56 1},
  pages={85-92}
}
Production of structure-grade mammalian membrane proteins in substantial quantities has been hindered by a lack of methods for effectively profiling multiple constructs expression in higher eukaryotic systems such as insect or mammalian cells. To address this problem, a specialized small-scale eukaryotic expression platform by Thomson Instrument Company (Vertiga-IM) was developed and used in tandem with a Guava EasyCyte microcapillary 96-well cytometer to monitor cell density and health and… CONTINUE READING

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