Profiling human breast epithelial cells using single cell RNA sequencing identifies cell diversity

@article{Nguyen2018ProfilingHB,
  title={Profiling human breast epithelial cells using single cell RNA sequencing identifies cell diversity},
  author={Q. Nguyen and Nicholas Pervolarakis and Kerrigan Blake and D. Ma and Ryan T. Davis and Nathan James and Anh Phung and E. Willey and Raj Kumar and E. Jabart and Ian H Driver and J. Rock and A. Goga and Seema A. Khan and D. Lawson and Z. Werb and K. Kessenbrock},
  journal={Nature Communications},
  year={2018},
  volume={9}
}
Breast cancer arises from breast epithelial cells that acquire genetic alterations leading to subsequent loss of tissue homeostasis. Several distinct epithelial subpopulations have been proposed, but complete understanding of the spectrum of heterogeneity and differentiation hierarchy in the human breast remains elusive. Here, we use single-cell mRNA sequencing (scRNAseq) to profile the transcriptomes of 25,790 primary human breast epithelial cells isolated from reduction mammoplasties of seven… Expand
Single-Cell RNA Sequencing of a Postmenopausal Normal Breast Tissue Identifies Multiple Cell Types That Contribute to Breast Cancer
TLDR
To assess which cell type(s) potentially contributes to breast cancer, the differential gene expression signature of each cell cluster was used to derive gene set variation analysis (GSVA) scores and classified breast tumors in The Cancer Gene Atlas dataset by assigning the highest GSVA scoring cell cluster number for each tumor. Expand
A single cell atlas of the healthy breast tissues reveal clinically relevant clusters of breast epithelial cells
TLDR
This study elucidates different epithelial cell types of the normal breasts and identifies a minor subpopulation of cells from which the majority of breast cancers may originate and suggests multiple cell-of-origin for an intrinsic subtype of breast cancer, including for hormone responsive breast cancers. Expand
A single-cell atlas of the healthy breast tissues reveals clinically relevant clusters of breast epithelial cells
TLDR
A rapid tissue collection/processing protocol is reported to perform scRNA-seq of breast biopsies of healthy women and 23 breast epithelial cell clusters are identified, identifying TBX3 and PDK4 as genes co-expressed with estrogen receptor (ER) in the normal breasts. Expand
Mammary cell gene expression atlas links epithelial cell remodeling events to breast carcinogenesis
TLDR
A mammary cell atlas derived from mouse and human single-cell RNA-sequence data is presented, characterize key life stages in which the mammary gland undergoes complex remodeling and, using breast cancer expression data, infer cells of origin for carcinogenesis of various breast cancer subtypes. Expand
Integrating single-cell RNA-sequencing and functional assays to decipher mammary cell states and lineage hierarchies
TLDR
ScRNA-seq studies are discussed in the context of previous research in which mammary epithelial cell populations were molecularly and functionally characterized, and c-Kit + progenitors and cell states analogous to those reported in the recent scRNA- sequencing studies are identified. Expand
Single-cell RNA-sequencing reveals distinct patterns of cell state heterogeneity in mouse models of breast cancer
TLDR
Through single-cell RNA-sequencing, it is found that Neu, PyMT and BRCA1-null mammary tumors each corresponded to a spectrum of minimally overlapping cell differentiation states without a universal BCSC population. Expand
Single cell transcriptome atlas of mouse mammary epithelial cells across development
  • B. Pal, Yunshun Chen, +10 authors J. Visvader
  • Medicine
  • Breast cancer research : BCR
  • 2021
TLDR
This extended single cell transcriptome atlas of mouse mammary epithelial cells provides the most complete coverage for mammaries during morphogenesis to date and represents a valuable framework for understanding developmental decisions within the mouse mammaries. Expand
High-throughput surface marker screen on primary human breast tissues reveals further cellular heterogeneity
TLDR
A dataset of CD marker expression in the normal breast provides better definition for breast cellular heterogeneity, and identifies 35 surface marker proteins expressed on normal breast epithelial and/or stromal subpopulations that were previously unreported. Expand
Aging-Associated Alterations in Mammary Epithelia and Stroma Revealed by Single-Cell RNA Sequencing
TLDR
A rich single-cell atlas of young and aged murine mammary tissues reveals the effects of aging on mammary physiology and can serve as a useful resource for understanding aging-associated cancer risk. Expand
Hybrid Stem Cell States: Insights Into the Relationship Between Mammary Development and Breast Cancer Using Single-Cell Transcriptomics
TLDR
Pseudotime analysis and alignment of TCGA breast cancer expression data revealed that breast cancer subtypes express distinct developmental signatures, with basal tumors representing the most “developmentally immature” phenotype. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 46 REFERENCES
Transcriptome analyses of mouse and human mammary cell subpopulations reveal multiple conserved genes and pathways
TLDR
The conservation of genes and pathways across species further validates the use of the mouse as a model to study mammary gland development and highlights pathways that are likely to govern cell-fate decisions and differentiation. Expand
Single-Cell RNA Sequencing Identifies Extracellular Matrix Gene Expression by Pancreatic Circulating Tumor Cells
TLDR
Mouse as well as human pancreatic CTCs exhibit a very high expression of stromal-derived extracellular matrix (ECM) proteins, including SPARC, whose knockdown in cancer cells suppresses cell migration and invasiveness, pointing to their contribution of microenvironmental signals for the spread of cancer to distant organs. Expand
A mammary stem cell population identified and characterized in late embryogenesis reveals similarities to human breast cancer.
TLDR
This work identifies, isolate, and characterize the fetal mammary stem cell (fMaSC) state since the invasive and proliferative processes of mammogenesis resemble phases of cancer progression, and reveals links between development and cancer and provides resources to identify new candidates for diagnosis, prognosis, and therapy. Expand
Myoepithelial Cells: Their Origin and Function in Breast Morphogenesis and Neoplasia
TLDR
Data from the laboratories and from others regarding the cellular origin of human myoepithelial cells, their function in maintaining tissue polarity in the normal breast, and their role during neoplasia are discussed. Expand
Single-cell RNA-seq highlights intratumoral heterogeneity in primary glioblastoma
TLDR
The genome sequence of single cells isolated from brain glioblastomas was examined, which revealed shared chromosomal changes but also extensive transcription variation, including genes related to signaling, which represent potential therapeutic targets. Expand
Phenotypic and functional characterisation of the luminal cell hierarchy of the mammary gland
TLDR
The Luminal cell compartment in the mammary epithelium is more heterogeneous than initially perceived since progenitors of varying levels of luminal cell differentiation and proliferative capacities can be identified. Expand
Construction of developmental lineage relationships in the mouse mammary gland by single-cell RNA profiling
TLDR
Findings point to a developmental hierarchy in which a basal-like gene expression program prevails in the early post-natal gland prior to the specification of distinct lineage signatures, and the presence of cellular intermediates that may serve as transit or lineage-primed cells. Expand
Characterization of bipotent mammary epithelial progenitor cells in normal adult human breast tissue
TLDR
A hierarchical branching model of HBEC progenitor differentiation from a primitive uncommitted cell to luminal- and myoepithelial-restricted progenitors is supported. Expand
Dissecting the multicellular ecosystem of metastatic melanoma by single-cell RNA-seq
TLDR
The cellular ecosystem of tumors is begin to unravel and how single-cell genomics offers insights with implications for both targeted and immune therapies is unraveled. Expand
Single-cell analysis reveals a stem-cell program in human metastatic breast cancer cells
TLDR
It is shown at the single-cell level that early stage metastatic cells possess a distinct stem-like gene expression signature, which supports a hierarchical model for metastasis, in which metastases are initiated by stem- like cells that proliferate and differentiate to produce advanced metastatic disease. Expand
...
1
2
3
4
5
...