Profiling cellular myristoylation and palmitoylation using ω-alkynyl fatty acids.

Abstract

Methods to detect and characterize cellular protein myristoylation and palmitoylation are invaluable in cell biology, immunology, and virology. Recently, we developed ω-alkynyl fatty acid probes for monitoring myristoylation and palmitoylation of cellular proteins. This article describes a biochemical procedure for metabolic labeling of cells with ω-alkynyl fatty acids and click chemistry.

DOI: 10.1007/978-1-61779-349-3_7
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@article{Hannoush2012ProfilingCM, title={Profiling cellular myristoylation and palmitoylation using ω-alkynyl fatty acids.}, author={Rami N. Hannoush}, journal={Methods in molecular biology}, year={2012}, volume={800}, pages={85-94} }