Profiling RNA interference (RNAi)-mediated toxicity in neural cultures for effective short interfering RNA design.

@article{Read2009ProfilingRI,
  title={Profiling RNA interference (RNAi)-mediated toxicity in neural cultures for effective short interfering RNA design.},
  author={Martin L Read and Sohaib Mir and Rachel Spice and Ruth J Seabright and Ellen L. Suggate and Zubair Ahmed and Martin Foster Berry and Ann Logan},
  journal={The journal of gene medicine},
  year={2009},
  volume={11 6},
  pages={
          523-34
        }
}
BACKGROUND A promising strategy to enhance axon regeneration is to employ short interfering (si)RNA targeting either RhoA or p75(NTR), which are components of a signalling cascade triggered by growth inhibitory ligands. However, it is important to profile the biological impact of siRNA on cell homeostasis in order to develop safe and effective therapies. METHODS We used microarray and quantitative reverse transcriptase-polymerase chain reaction techniques to analyse the transcriptional… CONTINUE READING
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