Products of reaction catalyzed by purified rat liver guanylate cyclase determined by 31p NMR spectroscopy.

@article{Tsai1980ProductsOR,
  title={Products of reaction catalyzed by purified rat liver guanylate cyclase determined by 31p NMR spectroscopy.},
  author={Shiou chuan Tsai and Heisaburo Shindo and Vincent C. Manganiello and Ronald Adamik and Martha Vaughan},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1980},
  volume={77 10},
  pages={
          5734-7
        }
}
Products of the reactions catalyzed by highly purified preparations of soluble guanylate cyclase [GTP pyrophosphate-lyase (cyclizing), EC 4.6.1.2] from rat liver were identified and quantified with 31P NMR spectroscopy. Utilization of this technique necessitated modification of the standard assay conditions; higher concentrations of enzyme and substrate (2 mM), Mg2+ instead of Mn2+, and longer incubation times (up to 46 hr) at 30 degrees C were used. Revision of our reported procedure for… CONTINUE READING
BETA