Production of triacylglycerols in Escherichia coli by deletion of the diacylglycerol kinase gene and heterologous overexpression of atfA from Acinetobacter baylyi ADP1

@article{Janen2013ProductionOT,
  title={Production of triacylglycerols in Escherichia coli by deletion of the diacylglycerol kinase gene and heterologous overexpression of atfA from Acinetobacter baylyi ADP1},
  author={Helge Jans Jan\ssen and Alexander Steinb{\"u}chel},
  journal={Applied Microbiology and Biotechnology},
  year={2013},
  volume={98},
  pages={1913-1924}
}
This study investigated the production of triacylglycerols in cells of the wild type of Escherichia coli and of a strain with a deleted diacylglycerol kinase gene (dgkA). By overexpression of atfA from Acinetobacter baylyi ADP1 and fadD from E. coli in the dgkA deletion mutant, cellular contents of up to 4.9 % (w/w) triacylglycerols could be achieved in batch cultivation. Furthermore, heterologous expression of atfA relieves the negative effects of dgkA deletion on growth. Process optimization… CONTINUE READING

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Osmotic regulation and the biosynthesis of membrane-derived oligosaccharides in Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America • 1982
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