Production of transgenic pigs harboring the human erythropoietin (hEPO) gene using somatic cell nuclear transfer.

@article{Cho2009ProductionOT,
  title={Production of transgenic pigs harboring the human erythropoietin (hEPO) gene using somatic cell nuclear transfer.},
  author={Seong-Keun Cho and Kyu-Chan Hwang and Yun-Jung Choi and H A Bui and Van Thuan Nguyen and Changkyu Park and Jae-Hwan Kim and Jin-Hoi Kim},
  journal={The Journal of reproduction and development},
  year={2009},
  volume={55 2},
  pages={128-36}
}
The production of transgenic pigs using somatic cell nuclear transfer (scNT) has been widely described, but a technique for removing nontransfected donor cells and for creating different founder animals has not yet been fully elucidated. In this study, four different expression vectors (pBC1hEPO, pMARBC1hEPO, pBC1hEPOwpre and pMARBC1hEPOwpre) were compared to determine the highest transgene expression, ideal conditions of enrichment of recombinant cells in vitro and efficiency of transgenesis… CONTINUE READING

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