Production of transgenic pea (Pisum sativum L.) plants by Agrobacterium tumefaciens — mediated gene transfer

@article{PuontiKaerlas1990ProductionOT,
  title={Production of transgenic pea (Pisum sativum L.) plants by Agrobacterium tumefaciens — mediated gene transfer},
  author={Johanna Puonti-Kaerlas and Tage Eriksson and P. -E. Engstr{\"o}m},
  journal={Theoretical and Applied Genetics},
  year={1990},
  volume={80},
  pages={246-252}
}
A transformation system that allows regeneration of transgenic pea plants from calli selected for antibiotic resistance was developed. Explants from axenic shoot cultures and seedling epicotyls were cocultivated with nononcogenic Agrobacterium tumefaciens strains, and transformed callus could be selected on callus-inducing media containing either 15 mg/l… CONTINUE READING

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