Production of transgenic gentian plants by particle bombardment of suspension-culture cells

  title={Production of transgenic gentian plants by particle bombardment of suspension-culture cells},
  author={Keizo Hosokawa and Rikyu Matsuki and Yayoi Oikawa and Saburo Yamamura},
  journal={Plant Cell Reports},
 Cell suspension cultures were established from leaf explants of gentian (Gentiana triflora×G. scabra) for the generation of transgenic plants by particle bombardment. The parameters for the bombardment of suspension culture cells with a particle gun were examined by monitoring the transient expression of a gene for β-glucuronidase driven by the cauliflower mosaic virus (CaMV) 35S promoter. We found that prior culture of suspension culture cells for 5 days on solid medium was optimum for… CONTINUE READING
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