Production of three new grapefruit cybrids with potential for improved citrus canker resistance

@article{Omar2017ProductionOT,
  title={Production of three new grapefruit cybrids with potential for improved citrus canker resistance},
  author={Ahmad Alsayed Omar and Mayara Mari Murata and Qibin Yu and Frederick G. Gmitter and Christine D. Chase and James H. Graham and Jude W. Grosser},
  journal={In Vitro Cellular \& Developmental Biology - Plant},
  year={2017},
  volume={53},
  pages={256-269}
}
Cybrid production, combining the nucleus of one species with alien cytoplasmic organelles of another, is a potentially valuable method used for improvement of various crops including Citrus species. [] Key Method’ In an effort to generate new grapefruit cultivars with enhanced canker resistance, putative cybrid grapefruit plants morphologically equivalent to standard grapefruit from all three combinations were produced. Four mitochondrial (mt) introns, a mt ribosomal RNA spacer region, and four chloroplast…
10 Citations
Novel Plastid-Nuclear Genome Combinations Enhance Resistance to Citrus Canker in Cybrid Grapefruit
TLDR
Cybridization was used to create cybrids between citrus canker resistant ‘Meiwa’ kumquat (Fortunella crassifolia Swingle snym) and susceptible grapefruit (Citrus paradisi Macfad) cultivars to serve as models for understanding the contribution of chloroplasts to plant disease response and raise the question of whether other alien chloroplast genotypes would condition similar results.
Molecular Characterization and Stress Tolerance Evaluation of New Allotetraploid Somatic Hybrids Between Carrizo Citrange and Citrus macrophylla W. rootstocks
TLDR
Two allotetraploid somatic hybrids recovered from the citrus rootstocks were characterized for morphology, genome composition using molecular markers, and their tolerance to iron chlorosis, salinity, and Citrus tristeza virus, indicating that losses and rearrangements occurred during the fusion process.
An integrated proteomic and metabolomic study to evaluate the effect of nucleus-cytoplasm interaction in a diploid citrus cybrid between sweet orange and lemon
TLDR
This investigation revealed differentially represented protein patterns that can be associated with genome rearrangement and cell compartment interplay in cybrids, and most of the up-regulated proteins in the cybrid are involved in crucial biological processes such as photosynthesis, energy production and stress tolerance response.
Grapefruit: History, Use, and Breeding
Grapefruit [Citrus ×aurantium (synonym C. ×paradisi)] is an important citrus commodity that originated in Barbados in the 17th century. Grapefruit is the youngest member of the genus Citrus. Most
Genomic Instability in Somatic Hybridization between Poncirus and Citrus Species Aiming to Create New Rootstocks
TLDR
Rootstocks are an important component for citrus adaptation to increasing biotic and abiotic stresses resulting from global climate change and symmetrical somatic hybridization between complementary diploid rootstocks of these two genera appears to be an efficient way to develop new tetraploidRootstocks in order to address the new challenges of the citrus industry.
Utilization of somatic fusion techniques for the development of HLB tolerant breeding resources employing the Australian finger lime (Citrus australasica)
TLDR
Allotetraploid finger lime hybrids and cybrids are developed by utilizing somatic cell fusion techniques to fuse diploid ‘OLL8’ sweet orange or ‘Page’ tangelo callus-derived protoplasts with finger lime (FL) mesophyll-derivedprotoplasts.
Incidence of Asiatic Citrus Canker on Trifoliate Orange and Its Hybrid Accessions in a Florida Field Planting
TLDR
Overall, hybrids of C. trifoliata with other citrus types displayed markedly reduced ACC sensitivity compared with C.trifolata, indicating that this trait is readily overcome through breeding.
Citrus biotechnology
Plant Tissue Culture and DNA Delivery Methods
TLDR
Transgenic plants are developed through gene delivery into single cell/tissue, regeneration into whole plants that are fertile and adapt to the normal plant propagation process and Gene transfer techniques can greatly reduce the time taken in crop improvement against conventional breeding procedures and enlarge the array for desired plant improvement.
Advances in Plant Transgenics: Methods and Applications
TLDR
Transgenic plants are developed through gene delivery into single cell/tissue, regeneration into whole plants that are fertile and adapt to the normal plant propagation process and Gene transfer techniques can greatly reduce the time taken in crop improvement against conventional breeding procedures and enlarge the array for desired plant improvement.

References

SHOWING 1-10 OF 68 REFERENCES
Cybridization of Grapefruit with ‘Dancy’ Mandarin Leads to Improved Fruit Characteristics
TLDR
These plants provide an opportunity to understand the contributions of cytoplasmic organelle genetics to important citrus fruit-breeding objectives and associated novel nuclear–organelle genome combinations with desirable fruit traits promising new market opportunities for Florida citrus.
Further evidence of a cybridization requirement for plant regeneration from citrus leaf protoplasts following somatic fusion
TLDR
The results support previous reports indicating that acquisition of the mitochondria of embryogenic protoplast by leaf protoplasts is a prerequisite for recovering plants with the leaf parent phenotype via somatic embryogenesis following somatic fusion.
Targeted cybridization in citrus: transfer of Satsuma cytoplasm to seedy cultivars for potential seedlessness
TLDR
This is the first report of targeted citrus cybrid production by symmetric fusion with male-sterile Satsuma as the callus parent and other seedy cultivars as the leaf parents.
APPLICATIONS OF SOMATIC HYBRIDIZATION AND CYBRIDIZATION IN SCION AND ROOTSTOCK IMPROVEMENT, WITH FOCUS ON CITRUS
TLDR
Applications for citrus scion improvement target the development of improved seedless fresh fruit varieties, and include symmetric somatic hybridization to produce superior alloteteraploid breeding parents and targeted cybridization to transfer mtCMS from Satsuma mandarin to seedy but otherwise desirable diploid cultivars.
Somatic hybridization in citrus: An effective tool to facilitate variety improvement
TLDR
Citrus somatic hybridization and cybridization via protoplast fusion has become an integral part of citrus variety improvement programs worldwide and is expected to have a positive impact on citrus cultivar improvement efforts.
Recovery of citrus cybrid plants with diverse mitochondrial and chloroplastic genome combinations by protoplast fusion followed by in vitro shoot, root, or embryo micrografting
TLDR
Plants were regenerated at high efficiency using in vitro micrografting of shoots, roots, and embryos recovered after different somatic hybridizations and exhibited a range of mitochondrial and chloroplastic genome combinations.
Non-random inheritance of mitochondrial genomes in Citrus hybrids produced by protoplast fusion
TLDR
Diploid plants with the nuclear profile of the mesophyll parent originated systematically from cells formed through spontaneous association of the nuclear genome of the Mesophyllparent and the mitochondrial genome ofThe embryogenic parent.
Efficient production of marker-free transgenic ‘Tarocco’ blood orange (Citrus sinensis Osbeck) with enhanced resistance to citrus canker using a Cre/loxP site-recombination system
TLDR
In this study, a transformation vector pLI35SAAT, harboring a Cre/loxP-mediated recombination system combined with the isopentenyl transferase (ipt) selectable marker gene and an anti-bacterial peptide gene AATCB was used to produce marker-free transgenic ‘Tarocco’ blood orange with enhanced resistance to citrus canker.
Plant Regeneration of `Valencia' Sweet Orange, `Femminello' Lemon, and the Interspecific Somatic Hybrid following Protoplasm Fusion
TLDR
The regeneration of plants from lemon leaf protoplasts is an example of protoplast-to -plant regeneration from non-nucellus-derived tissue for Citrus and could increase the number of totipotent Citrus clones amenable to somatic hybrids and genetic transformation experiments.
...
...