Production of specific antisera and monoclonal antibodies to choline acetyltransferase: characterization and use for identification of cholinergic neurons.

@article{Eckenstein1982ProductionOS,
  title={Production of specific antisera and monoclonal antibodies to choline acetyltransferase: characterization and use for identification of cholinergic neurons.},
  author={Felix P. Eckenstein and Hans Thoenen},
  journal={The EMBO journal},
  year={1982},
  volume={1 3},
  pages={
          363-8
        }
}
  • Felix P. Eckenstein, Hans Thoenen
  • Published in The EMBO journal 1982
  • Biology, Medicine
  • Choline acetyltransferase (ChAT) has been purified from pig brain to greater than 95% homogeneity (purification factor: 646 000, specific activity of the purified enzyme: 128 mumol acetylcholine formed/min/mg). Gel electrophoresis of the purified enzyme in the presence of sodium dodecylsulphate and beta-mercaptoethanol revealed a single protein band at 68 000 daltons. Immunoprecipitation and double immunodiffusion tests showed that antisera raised against this protein specifically recognize… CONTINUE READING

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