Production of soluble mammalian proteins in Escherichia coli: identification of protein features that correlate with successful expression

Abstract

BACKGROUND In the search for generic expression strategies for mammalian protein families several bacterial expression vectors were examined for their ability to promote high yields of soluble protein. Proteins studied included cell surface receptors (Ephrins and Eph receptors, CD44), kinases (EGFR-cytoplasmic domain, CDK2 and 4), proteases (MMP1, CASP2… (More)

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@article{Dyson2004ProductionOS, title={Production of soluble mammalian proteins in Escherichia coli: identification of protein features that correlate with successful expression}, author={Michael R. Dyson and S. Paul Shadbolt and Karen J Vincent and Rajika L. Perera and John Mccafferty}, journal={BMC Biotechnology}, year={2004}, volume={4}, pages={32 - 32} }