Production of soluble and functional engineered antibodies in Escherichia coli improved by FkpA.

@article{Zhang2003ProductionOS,
  title={Production of soluble and functional engineered antibodies in Escherichia coli improved by FkpA.},
  author={Zhong Zhang and Li-ping Song and Min Fang and Fei Wang and Dan He and Rui Zhao and Jing Liu and Zhi-yong Zhou and Chang-cheng Yin and Qing Lin and Hua-liang Huang},
  journal={BioTechniques},
  year={2003},
  volume={35 5},
  pages={
          1032-8, 1041-2
        }
}
Overproduction of genetically engineered antibodies, such as single-chain antibodies (scAbs) in Escherichia coli often results in insoluble and inactive products known as inclusion bodies. We now report that fusion or co-expression of FkpA, the E. coli periplasmic peptidyl-prolyl-isomerase with chaperone activity, substantially improves soluble and functional expression of scAbs. Anti-human bladder carcinoma scAb (PG) and anti-human CD3 x anti-human ovarian carcinoma-bispecific scAb (BH1) were… 
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