Production of single chain fragment variable (scFv) antibodies in Escherichia coli using the LEX™ bioreactor.

@article{Miethe2013ProductionOS,
  title={Production of single chain fragment variable (scFv) antibodies in Escherichia coli using the LEX™ bioreactor.},
  author={Sebastian Miethe and Torsten Meyer and Stefanie W{\"o}hl-Bruhn and Andr{\'e} Frenzel and Thomas Schirrmann and Stefan D{\"u}bel and Michael Hust},
  journal={Journal of biotechnology},
  year={2013},
  volume={163 2},
  pages={105-11}
}
For proteome research, antibodies against a growing number of antigens must be generated and characterized. The high throughput generation of antibody fragments, using in vitro selection, requires bacterial expression of antibody fragments. This created a need to establish an expression method to improve the parallel production of many antibody fragments. In this study, we describe the development of a high throughput bacterial production method for single chain fragment variables (scFvs) using… CONTINUE READING
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