Production of recombinant proteins in high-density insect cell cultures.

@article{Reuveny1993ProductionOR,
  title={Production of recombinant proteins in high-density insect cell cultures.},
  author={S. Reuveny and Yoo Jeong Kim and Christopher W. Kemp and Joseph Shiloach},
  journal={Biotechnology and bioengineering},
  year={1993},
  volume={42 2},
  pages={235-9}
}
The effect of the growth phase of Spodoptera frugiperda (Sf9) cells on the production of recombinant proteins (beta-galactosidase and glucocerebrosidase) was investigated. Cells infected with the recombinant Autographa californica nuclear polyhedrosis virus at the late exponential and stationary phases yielded low quantities of expressed protein. Highest enzyme yields were obtained using Sf9 cells from the early exponential phase (0.9 mg beta-galactosidase/10(6) cells and 1.7 microg… CONTINUE READING

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