Production of recombinant proteins by microbes and higher organisms.

@article{Demain2009ProductionOR,
  title={Production of recombinant proteins by microbes and higher organisms.},
  author={Arnold L. Demain and Preeti Vaishnav},
  journal={Biotechnology advances},
  year={2009},
  volume={27 3},
  pages={297-306}
}
Large proteins are usually expressed in a eukaryotic system while smaller ones are expressed in prokaryotic systems. For proteins that require glycosylation, mammalian cells, fungi or the baculovirus system is chosen. The least expensive, easiest and quickest expression of proteins can be carried out in Escherichia coli. However, this bacterium cannot express very large proteins. Also, for S-S rich proteins, and proteins that require post-translational modifications, E. coli is not the system… CONTINUE READING
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