Production of recombinant proteins: challenges and solutions.

@article{Palomares2004ProductionOR,
  title={Production of recombinant proteins: challenges and solutions.},
  author={Laura A. Palomares and Sandino Estrada-Mondaca and Octavio T. Ram{\'i}rez},
  journal={Methods in molecular biology},
  year={2004},
  volume={267},
  pages={
          15-52
        }
}
Efficient strategies for the production of recombinant proteins are gaining increasing importance, as more applications that require high amounts of high-quality proteins reach the market. Higher production efficiencies and, consequently, lower costs of the final product are needed for obtaining a commercially viable process. In this chapter, common problems in recombinant protein production are reviewed and strategies for their solution are discussed. Such strategies include molecular biology… 
Recombinant protein expression in Escherichia coli: advances and challenges
TLDR
The different approaches for the synthesis of recombinant proteins in E. coli are reviewed and recent progress in this ever-growing field is discussed.
Application of Continuous Culture Methods to Recombinant Protein Production in Microorganisms
TLDR
This review summarizes the uses of continuous cultures in cell physiology studies and process development, with a focus on recombinant protein-producing microorganisms.
Leishmania-based expression systems
TLDR
New Leishmania-based expression systems are compared with current systems that have long histories in research and industry.
PRODUCCIÓN DE PROTEÍNAS RECOMBINANTES EN Escherichia coli RECOMBINANT PROTEIN PRODUCTION IN Escherichia coli
TLDR
General aspects of vector design, strain selection as well as cultivation and downstream processing are covered, and the opportunities to improve cultivation schemes and downstream through metabolic engineering are discussed.
Fusion tags for protein solubility, purification and immunogenicity in Escherichia coli: the novel Fh8 system
TLDR
An overview of current strategies to improve recombinant protein production in E. coli is provided, including the key factors for successful protein production, highlighting solubleprotein production, and a comprehensive summary of the latest available and traditionally used gene fusion technologies.
Application of the enzyme controlled glucose feeding for the production of recombinant proteins
TLDR
A scalability study for the enzymatic glucose feeding system (Enpresso B growth system) was performed with the optimized conditions using a nucleoside phosphorylase (NP) as a model protein and showed a more than three-fold increase in the protein activity and a 30% increase of the cell density from the well plate scale to the shake flask level.
Engineering of protein secretion in yeast: strategies and impact on protein production
TLDR
This mini-review focuses on recent strategies and their advantages for systematic engineering of yeast strains for effective protein secretion.
The transgenic animal platform for biopharmaceutical production
TLDR
The approval of two mammary gland-derived recombinant proteins for commercial and clinical use has boosted the interest for more efficient, safer and economic ways to generate transgenic founders to meet the increasing demand for biomedical proteins worldwide.
The mammary gland : bioreactor for the production of recombinant proteins
TLDR
In this review, the main advantages and drawbacks of the currently available expression systems are discussed, stressing the potential of the mammary gland as a biofactory.
...
...

References

SHOWING 1-10 OF 189 REFERENCES
Advances in Escherichia coli production of therapeutic proteins.
  • J. Swartz
  • Biology
    Current opinion in biotechnology
  • 2001
Recombinant protein expression for therapeutic applications.
Efficient production of secreted proteins by Aspergillus : progress, limitations and prospects
TLDR
This review will focus on the use of filamentous fungi for the production of heterologous proteins, especially non-fungal, proteins and the effect of gene-fusion strategies will be reviewed.
Strategies for optimizing heterologous protein expression in Escherichia coli.
Genetic optimization of recombinant glycoprotein production by mammalian cells.
Genetic design for facilitated production and recovery of recombinant proteins in Escherichia coli
TLDR
It is evident that genetic strategies will be of utmost importance in the future for facilitating production and recovery of recombinant proteins.
Recombinant protein expression in Escherichia coli.
  • F. Baneyx
  • Biology
    Current opinion in biotechnology
  • 1999
Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris.
Understanding the art of producing protein and nonprotein molecules in Escherichia coli
TLDR
This review presents a compilation of the basic issues that influence the success in the production of protein and nonprotein products in Escherichia coli, as well as some general strategies designed to facilitate downstream process operations and improve biosynthesis yields.
...
...