Production of recombinant proteins: challenges and solutions.
@article{Palomares2004ProductionOR, title={Production of recombinant proteins: challenges and solutions.}, author={Laura A. Palomares and Sandino Estrada-Mondaca and Octavio T. Ram{\'i}rez}, journal={Methods in molecular biology}, year={2004}, volume={267}, pages={ 15-52 } }
Efficient strategies for the production of recombinant proteins are gaining increasing importance, as more applications that require high amounts of high-quality proteins reach the market. Higher production efficiencies and, consequently, lower costs of the final product are needed for obtaining a commercially viable process. In this chapter, common problems in recombinant protein production are reviewed and strategies for their solution are discussed. Such strategies include molecular biology…
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References
SHOWING 1-10 OF 189 REFERENCES
Advances in Escherichia coli production of therapeutic proteins.
- BiologyCurrent opinion in biotechnology
- 2001
Recombinant protein expression for therapeutic applications.
- BiologyCurrent opinion in biotechnology
- 2002
Efficient production of secreted proteins by Aspergillus : progress, limitations and prospects
- Biology, EngineeringApplied Microbiology and Biotechnology
- 1997
This review will focus on the use of filamentous fungi for the production of heterologous proteins, especially non-fungal, proteins and the effect of gene-fusion strategies will be reviewed.
Strategies for optimizing heterologous protein expression in Escherichia coli.
- BiologyTrends in biotechnology
- 1998
Genetic optimization of recombinant glycoprotein production by mammalian cells.
- Biology, EngineeringTrends in biotechnology
- 1999
Filamentous fungi as cell factories for heterologous protein production.
- BiologyTrends in biotechnology
- 2002
Genetic design for facilitated production and recovery of recombinant proteins in Escherichia coli
- Biology, EngineeringBiotechnology and applied biochemistry
- 2002
It is evident that genetic strategies will be of utmost importance in the future for facilitating production and recovery of recombinant proteins.
Recombinant protein expression in Escherichia coli.
- BiologyCurrent opinion in biotechnology
- 1999
Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris.
- Biology, EngineeringCurrent opinion in biotechnology
- 2002
Understanding the art of producing protein and nonprotein molecules in Escherichia coli
- BiologyMolecular biotechnology
- 2001
This review presents a compilation of the basic issues that influence the success in the production of protein and nonprotein products in Escherichia coli, as well as some general strategies designed to facilitate downstream process operations and improve biosynthesis yields.