Production of recombinant human relaxin 3 in AtT20 cells.

  title={Production of recombinant human relaxin 3 in AtT20 cells.},
  author={Hideki Kizawa and Kazunori Nishi and Yoshihiro Ishibashi and Masataka Harada and Tsuneo Asano and Yasuaki Ito and Nobuhiro Suzuki and Shuji Hinuma and Yukio Fujisawa and Haruo Onda and Osamu Nishimura and Masahiko Fujino},
  journal={Regulatory peptides},
  volume={113 1-3},
Relaxin 3 has been reported recently as a member of the insulin/IGF/relaxin family. To clarify the function of relaxin 3, we prepared recombinant human relaxin 3 using a mouse adrenocorticotrophic hormone (ACTH)-secreting cell line, AtT20. To detect a mature form of recombinant human relaxin 3, a competitive enzyme immunoassay (EIA) was developed using a monoclonal antibody (mAb; HK4-144-10), which was raised for the N-terminal peptide of human relaxin 3 A-chain. We detected immunoreactive (ir… CONTINUE READING
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