Production of recombinant collagen: state of the art and challenges

@inproceedings{Wang2017ProductionOR,
  title={Production of recombinant collagen: state of the art and challenges},
  author={Tianyi Wang and J Lew and J. Premkumar and Chueh Loo Poh and May W Naing},
  year={2017}
}
Collagen, which is often used in healthcare materials and biomedical research, is largely extracted from animal sources. Recombinant human collagen has the potential to be a promising alternative to animal collagen which has many shortcomings, including immunogenicity and lack of biocompatibility. Currently, recombinant human collagen has been expressed in both eukaryotic and prokaryotic hosts with varying degrees of success. One issue with recombinant collagen across all hosts is the inability… 
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References

SHOWING 1-10 OF 102 REFERENCES

Shining light on collagen: expressing collagen in plants.

Progress toward plant production of collagens, the ability to self-assemble these recombinant proteins into higher-order structures, and the utility of these materials in various medical applications suggest an important path forward for the field.

Making recombinant extracellular matrix proteins.

Liquid crystalline human recombinant collagen: the challenge and the opportunity.

Recombinant human collagen produced in transgenic tobacco plants has the capacity of forming highly concentrated liquid crystalline dope that can be aligned by application of shear force, opening new possibilities toward obtaining scaffolds that may be able to provide the necessary mechanical support, while closely mimicking the molecular signals and mechanical cues displayed to natural cell milieu.

Recombinant expression systems for the production of collagen.

The application of various expression systems to procollagen production is outlined and the use of the mammary gland is detailed as a suitable bioreactor for the synthesis of significant amounts of novel procollagens from cDNA constructs.

Expression of recombinant human type I-III collagens in the yeast pichia pastoris.

Methods for producing the three major fibril-forming human collagens, types I, II and III, in the methylotrophic yeast Pichia pastoris are developed, based on co-expression of procollagen polypeptide chains with the alpha- and beta-subunits of prolyl 4-hydroxylase.

Functional and bioactive properties of collagen and gelatin from alternative sources: A review

Production of bioactive, post-translationally modified, heterotrimeric, human recombinant type-I collagen in transgenic tobacco.

Collagen's biocompatibility, biodegradability and low immunogenicity render it advantageous for extensive application in pharmaceutical or biotechnological disciplines. However, typical collagen…

Ultrarapid purification of collagen type I for tissue engineering applications.

A 3-h method to isolate COL1 from rabbit, lamb, and human skin in sufficient quantities by using a rapid agitation technique and incorporating centrifugal filtration units into a traditional isolation procedure increases the feasibility of autologous COL1 use in humans as well as overall safety for clinical patients.

Collagen interactions: Drug design and delivery.

Characterization of Recombinant Human Type IX Collagen

The results suggest that the most favored molecular species is an α1(ix)α2(IX)α3(IX), but the chains are also able to form disulfide-bonded heterotrimers of α1-IX and α3-IX chains and (α1(IX))3, (α2-IX)3, and ( α3(ix))3 homotriming.
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