Production of reactive oxygen species by spermatozoa undergoing cooling, freezing, and thawing.

@article{Chatterjee2001ProductionOR,
  title={Production of reactive oxygen species by spermatozoa undergoing cooling, freezing, and thawing.},
  author={Saurabh Chatterjee and Claude Gagnon},
  journal={Molecular reproduction and development},
  year={2001},
  volume={59 4},
  pages={451-8}
}
In the present study, we provide evidence for the production of reactive oxygen species (ROS) during cryopreservation of bovine spermatozoa. Cooling and thawing of spermatozoa cause an increase in the generation of superoxide radicals. Although nitric oxide production remains unaltered during sperm cooling from 22-4 degrees C, a sudden burst of nitric oxide radicals is observed during thawing. Increase in lipid peroxidation levels have been observed in frozen/thawed spermatozoa and appears to… CONTINUE READING
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