Production of reactive oxygen species by microsomes enriched in specific human cytochrome P450 enzymes.

@article{Puntarulo1998ProductionOR,
  title={Production of reactive oxygen species by microsomes enriched in specific human cytochrome P450 enzymes.},
  author={Susana Puntarulo and Arthur I. Cederbaum},
  journal={Free radical biology & medicine},
  year={1998},
  volume={24 7-8},
  pages={1324-30}
}
Few studies have evaluated the production of reactive oxygen intermediates by human microsomes, especially the influence of the specific form of cytochrome P450. Experiments were carried out to evaluate the ability of CYP1A1, 1A2, 2B6, and 3A4 to consume NADPH, reduce iron, and catalyze production of reactive oxygen species. Microsomes enriched in each of these CYPs were obtained from commercial +/- lymphoblast cells that had been transfected with cDNA encoding the specific human CYP. On a per… CONTINUE READING