• Biology
  • Published 2011

Production of polyclonal antibody against tetracycline using KLH as a carrier protein

@inproceedings{Noraini2011ProductionOP,
  title={Production of polyclonal antibody against tetracycline using KLH as a carrier protein},
  author={Siti Noraini and Nur Farah Azura},
  year={2011}
}
The production of polyclonal antibody against tetracycline was described using tetracycline - KLH conjugate (Keyhole Limpet Hemocyanin). Tetracycline was conjugated with KLH as a carrier protein because it was small molecule and unable to stimulate an immune response by itself. The UV absorbance reading of the tetracycline-KLH conjugate and KLH alone slightly shifted the reading of UV absorbance. Ammonium sulphate precipitation and Protein A affinity column were used in the antibody… CONTINUE READING

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