Production of pneumolysin, a pneumococcal toxin, in Bacillus subtilis.

@article{Taira1989ProductionOP,
  title={Production of pneumolysin, a pneumococcal toxin, in Bacillus subtilis.},
  author={Suvi Taira and E. Jalonen and James C Paton and Matti Sarvas and K Runeberg-Nyman},
  journal={Gene},
  year={1989},
  volume={77 2},
  pages={211-8}
}
We have expressed the pneumolysin gene of Streptococcus pneumoniae in Bacillus subtilis, both from its own promoter and as a fusion protein. The level of expression of pneumolysin from its own promoter was low. The protein produced was hemolytically active. A higher level of expression (about 10 micrograms/ml of culture) was achieved when either one of two C-terminal fragments (corresponding to amino acids 265-471 and 55-471, respectively) or the entire coding part of the pneumolysin gene were… CONTINUE READING