Production of nuclease-formine 5'-nucleotide by Aspergillus quercinus in a low phosphate medium.

@article{Ohta1968ProductionON,
  title={Production of nuclease-formine 5'-nucleotide by Aspergillus quercinus in a low phosphate medium.},
  author={Y. Ohta and S. Ueda},
  journal={Applied microbiology},
  year={1968},
  volume={16 9},
  pages={
          1293-9
        }
}
The production of ribonucleic acid (RNA)-depolymerase-forming 5'-nucleotides (5'-nuclease) was investigated with the fungus Aspergillus quercinus in media containing 68, 10, 5, 3, 1, and 0.5 mg of phosphorus per 100 ml. Yields were maximal with 5 mg of phosphorus per 100 ml. RNA-depolymerase-forming 3'-nucleosides (3'-nuclease) and phosphomonoesterase were maximal in media containing 1 and 0.5 mg of phosphorus per 100 ml. The 5'-nuclease was purified approximately 530-fold with a recovery of 84… Expand
3 Citations
[12] Increasing enzyme production by genetic and environmental manipulations
TLDR
This chapter focuses on several manipulations that can be used to modify, to bypass, or to utilize regulatory mechanisms to force “overproduction” of enzymes in the laboratory. Expand
Overproduction of microbial metabolites and enzymes due to alteration of regulation
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . l 13 1. Coordination of Microbial Metabolism . . . . . . . . . . . . . . . 114 a) Induction . . . . . . . . . . . . . . . . . . .Expand

References

SHOWING 1-8 OF 8 REFERENCES
Production of phosphatase by Aspergillus awamori var. kawachii in a low phosphate medium.
TLDR
The enzymes of phosphate metabolism appear to be activated by nonspecific phosphate sources, especially when various phosphate esters were used as substrates. Expand
Degradation of Nucleic Acids and their Related Compounds by Microbial Enzymes
TLDR
The distribution in microorganisms of extracellular enzymes which degrade DNA into deoxymononucleotides was studied and it was found that except Bacillus subtilis IFO 3302, the microorganisms which produced the enzymes that catalyze the degradation of RNA into 5′-monon nucleotides produced enzymes capable of hydrolyzing DNA. Expand
Determination of protein , p . 166
  • Methods in enzymology
  • 1955
Optical properties of nucleic acids and their components , p . 493 - 553
  • The nucleic acids
  • 1955
Purification and properties of 5-nucleotidase.