Production of nattokinase by high cell density fed-batch culture of Bacillus subtilis

@article{Kwon2011ProductionON,
  title={Production of nattokinase by high cell density fed-batch culture of Bacillus subtilis},
  author={Eun-Yeong Kwon and Kyung Mi Kim and Mi Kyoung Kim and In-Young Lee and Beom Soo Kim},
  journal={Bioprocess and Biosystems Engineering},
  year={2011},
  volume={34},
  pages={789-793}
}
Bacillus subtilis was cultivated to high cell density for nattokinase production by pH-stat fed-batch culture. A concentrated mixture solution of glucose and peptone was automatically added by acid-supplying pump when culture pH rose above high limit. Effect of the ratio of glucose to peptone in feeding solution was investigated on cell growth and nattokinase production by changing the ratio from 0.2 to 5 g glucose/g peptone. The highest cell concentration was 77 g/L when the ratio was 0.2 g… 
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34 Citations
Highly Influential Citations
3
Background Citations
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Methods Citations
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Results Citations
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34 Citations

High Cell Density Cultivation of A Recombinant Bacillus Subtilis for Nattokinase Production
TLDR
There is a balance between the cell growth rate and NK expression when recombinant Bacillus subtilis is cultured with a fed-batch process and the equilibrium state can be attained by optimizing the induction and feeding strategy, and thus a high cell density and enzyme activity can be achieved.
Optimized Fed-Batch Fermentation of Scheffersomyces stipitis for Efficient Production of Ethanol from Hexoses and Pentoses
TLDR
Higher efficiency ethanol production was achieved in this study through fed-batch process optimization, which may contribute to an improvement of ethanol fermentation from lignocellulosic biomass by S. stipitis on the industrial scale.
Optimization of the Fermentation Medium to Receive The Highest Biomass Yield By Bacillus Subtilis Natto And The Initial Test Of Nattokinase Yield
A bacterium, named Bacillus subtilis natto, is employed in medium optimization in order to be applied for nattokinase enzyme production. Six factors including glucose (X1), soybean peptone (X2),…
Fibrinolytic enzyme production from low-cost substrates by marine Bacillus subtilis: Process optimization and kinetic modeling
Feeding strategy design for recombinant human growth hormone production by Bacillus subtilis
TLDR
The results indicate the requirement of designing continuous feed stream in fed-batch production to enhance rhGH production by r-B.
Fed-Batch Biomolecule Production by Bacillus subtilis: A State of the Art Review.
Heterologous expression of nattokinase from B. subtilis natto using Pichia pastoris GS115 and assessment of its thrombolytic activity
TLDR
This study suggests that the P. pastoris expression system is an ideal platform for the large-scale, low-cost preparation of nattokinase.
Purification and Characterization of Nattokinase from Bacillus Subtilis from Coconut Field Soils
Nattokinase is an enzyme that finds a wide range of applications in pharmaceutical industry, health care and medicine etc. The main aim of research work is to screen and identification of Bacillus…
Optimization of Culture Conditions to Enhance Nattokinase Production Using RSM
This study was conducted with the aim to isolate, characterize and optimize of Bacillus subtilis that produce nattokinase with highest activity under optimal culture conditions. The study was carried…
Statistical Optimization of Culture Conditions for Nattokinase Production by Batch Fermentation
TLDR
Nattokinase was discovered in Natto, a fermented cheese-like food that has been used in Japan for over 1000 year, and is a potent fibrinolytic enzyme that finds a wide range of applications in Pharmaceutical industry, health care and Medicine.
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