Production of nattokinase by high cell density fed-batch culture of Bacillus subtilis

  title={Production of nattokinase by high cell density fed-batch culture of Bacillus subtilis},
  author={Eun-Yeong Kwon and Kyung Mi Kim and Mi Kyoung Kim and In-Young Lee and Beom Soo Kim},
  journal={Bioprocess and Biosystems Engineering},
Bacillus subtilis was cultivated to high cell density for nattokinase production by pH-stat fed-batch culture. A concentrated mixture solution of glucose and peptone was automatically added by acid-supplying pump when culture pH rose above high limit. Effect of the ratio of glucose to peptone in feeding solution was investigated on cell growth and nattokinase production by changing the ratio from 0.2 to 5 g glucose/g peptone. The highest cell concentration was 77 g/L when the ratio was 0.2 g… 
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Production of nattokinase by batch and fed-batch culture of Bacillus subtilis.
Production of poly(3‐hydroxybutyric acid) by fed‐batch culture of Alcaligenes eutrophus with glucose concentration control
The final cell concentration, PHB concentration, and PHB productivity increased as ammonia feeding was stopped at a higher cell concentration and the effect of ammonium limitation on PHB synthesis at different culture phases was studied.
High cell density fed-batch cultivation of Escherichia coli using exponential feeding combined with pH-stat
With this feeding strategy, recombinant Escherichia coli could be grown to 101 g/l by controlling the specific growth rate at 0.1 h−1.
Production of medium chain length polyhydroxyalkanoates by fed-batch culture of Pseudomonas oleovorans
  • B. Kim
  • Engineering, Biology
    Biotechnology Letters
  • 2004
Pseudomonas oleovorans was cultivated to produce medium chain length polyhydroxyalkanoates (MCL-PHAs) from octanoic acid and ammonium nitrate as carbon and nitrogen source, respectively, by a pH-stat
Growing E. coli to high cell density--a historical perspective on method development.
Production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) by Ralstonia eutropha
Strategy To Approach Stable Production of Recombinant Nattokinase in Bacillus subtilis
The feasibility of the approach by fusing two compatible plasmid origins for stable and efficient production of recombinant nattokinase in B. subtilis is indicated.