Production of native-type Streptoverticillium mobaraense transglutaminase in Corynebacterium glutamicum.

@article{Date2003ProductionON,
  title={Production of native-type Streptoverticillium mobaraense transglutaminase in Corynebacterium glutamicum.},
  author={Masayo Date and Kei-ichi Yokoyama and Yukiko Umezawa and Hiroshi Matsui and Yoshimi Kikuchi},
  journal={Applied and environmental microbiology},
  year={2003},
  volume={69 5},
  pages={
          3011-4
        }
}
We previously observed secretion of active-form transglutaminase in Corynebacterium glutamicum by coexpressing the subtilisin-like protease SAM-P45 from Streptomyces albogriseolus to process the prodomain. However, the N-terminal amino acid sequence of the transglutaminase differed from that of the native Streptoverticillium mobaraense enzyme. In the present work we have used site-directed mutagenesis to generate an optimal SAM-P45 cleavage site in the C-terminal region of the prodomain. As a… CONTINUE READING

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