Production of native creatine kinase B in insect cells using a baculovirus expression vector

@article{Kok1995ProductionON,
  title={Production of native creatine kinase B in insect cells using a baculovirus expression vector},
  author={Yvette J.M. de Kok and Monique P. A. Geurds and Erik A Sistermans and Magda Usmany and Just M. Vlak and B{\'e} Wieringa},
  journal={Molecular and Cellular Biochemistry},
  year={1995},
  volume={143},
  pages={59-65}
}
A full-length human creatine kinase B (B-CK) cDNA was used to produce a recombinant baculovirus (AcDZ1-BCK). Sf9 cells infected with this recombinant expressed a homodimeric protein composed of 43 kDa subunits which, under optimal conditions, formed up to 30% of the total soluble cellular protein. Upon analysis by PAGE, zymogram assay and gel filtration chromatography the recombinant protein behaved like authentic dimeric human BB-CK protein. Studies with a newly produced monoclonal antibody… CONTINUE READING

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