Production of motile acrosome-reacted mouse sperm with nanomolar concentration of calcium ionophore A23187.

@article{Tanphaichitr1994ProductionOM,
  title={Production of motile acrosome-reacted mouse sperm with nanomolar concentration of calcium ionophore A23187.},
  author={Nongnuj Tanphaichitr and Clare Hansen},
  journal={Molecular reproduction and development},
  year={1994},
  volume={37 3},
  pages={326-34}
}
A method to generate a population of motile, acrosome-reacted mouse sperm is described. Sperm retrieved from the cauda epididymis and vas deferens were first capacitated in a 3% bovine serum albumin (BSA) containing medium. Sperm were then resuspended in medium with low BSA content (0.01%) and treated with 30 nM of the calcium ionophore, A23187, which was added as a single dose of 30 nM for 15 min at 37 degrees C; or three sequential 10 nM doses over three 5 min intervals. Approximately 55-60… CONTINUE READING

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