• Biology, Medicine
  • Published in Diseases of aquatic organisms 2005
  • DOI:10.3354/dao063053

Production of monoclonal antibodies against grouper nervous necrosis virus (GNNV) and development of an antigen capture ELISA.

@article{Shieh2005ProductionOM,
  title={Production of monoclonal antibodies against grouper nervous necrosis virus (GNNV) and development of an antigen capture ELISA.},
  author={J R Shieh and S C Chi},
  journal={Diseases of aquatic organisms},
  year={2005},
  volume={63 1},
  pages={
          53-60
        }
}
Five (2 IgG, 3 IgM) monoclonal antibodies (MAbs) against the G9508KS strain of grouper nervous necrosis virus (GNNV) were produced and characterized. All 5 MAbs showed positive signals in the retina of GNNV-infected grouper larvae and in the cytoplasm of GNNV-infected GF-1 cells using immunohistochemistry staining. Two MAbs reacted with the denatured capsid protein derived from GNNV-infected GF-1 cells in Western blot analysis, but did not react with the GNNV recombinant capsid protein… CONTINUE READING

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