Production of maternal-zygotic mutant zebrafish by germ-line replacement.

  title={Production of maternal-zygotic mutant zebrafish by germ-line replacement.},
  author={Brian Ciruna and Gilbert Weidinger and Holger Knaut and Bernard V Thisse and Christine I Thisse and Erez Raz and Alexander F Schier},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={99 23},
We report a generally applicable strategy for transferring zygotic lethal mutations through the zebrafish germ line. By using a morpholino oligonucleotide that blocks primordial germ cell (PGC) development, we generate embryos devoid of endogenous PGCs to serve as hosts for the transplantation of germ cells derived from homozygous mutant donors. Successful transfers are identified by the localization of specifically labeled donor PGCs to the region of the developing gonad in chimeric embryos… CONTINUE READING


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