Production of male cloned mice from fresh, cultured, and cryopreserved immature Sertoli cells.

@article{Ogura2000ProductionOM,
  title={Production of male cloned mice from fresh, cultured, and cryopreserved immature Sertoli cells.},
  author={Atsuo Ogura and Kimiko Inoue and Narumi Ogonuki and Atsutaka Noguchi and Kaoru J. Takano and Reiko Nagano and On Suzuki and Jiyoung Lee and Fumitoshi Ishino and Junichiro Matsuda},
  journal={Biology of reproduction},
  year={2000},
  volume={62 6},
  pages={
          1579-84
        }
}
Although it is generally accepted that relatively high efficiencies of somatic cell cloning in mammals can be achieved by using donor cells from the female reproductive system (e.g., cumulus/granulosa, oviduct, and mammary gland cells), there is little information on the possibility of using male-specific somatic cells as donor cells. In this study we injected the nucleus of immature mouse Sertoli cells isolated from the testes of newborn (Days 3-10) males into enucleated mature oocytes in… CONTINUE READING

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