Production of lymphotoxin (LT alpha) and a soluble dimeric form of its receptor using the baculovirus expression system.

@article{Crowe1994ProductionOL,
  title={Production of lymphotoxin (LT alpha) and a soluble dimeric form of its receptor using the baculovirus expression system.},
  author={Paul D. Crowe and T L Vanarsdale and Barbara N Walter and Kimberly M. Dahms and Carl F Ware},
  journal={Journal of immunological methods},
  year={1994},
  volume={168 1},
  pages={79-89}
}
Human LT alpha and a fusion protein (p60:Fc) comprised of the extracellular domain of the 60 kDa TNF receptor (TNFR60) fused to the Fc portion of human IgG1 were produced in insect cells infected with recombinant baculoviruses. The p60:Fc fusion produced in insect cells accumulates in culture supernatants to levels > 2 mg/l. Purified p60:Fc binds human TNF and LT alpha with high affinity (200-600 pM) and neutralizes TNF cytolytic activity at equimolar stoichiometric concentration. The data show… CONTINUE READING

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