Production of lipase by clinical isolates of Pseudomonas cepacia.

  title={Production of lipase by clinical isolates of Pseudomonas cepacia.},
  author={Muhammed Lonon and Donald E. Woods and David C. Straus},
  journal={Journal of clinical microbiology},
  volume={26 5},
Ten clinical isolates of Pseudomonas cepacia from the sputum of cystic fibrosis patients were examined for the ability to produce lipase. Lipase substrates used included egg yolk agar, four different polyoxyethylene sorbitans (Tweens), and p-nitrophenylphosphorylcholine, a chromogenic substrate used to assay for phospholipase C. Lipase activity was detected in the filtrates of organisms grown to the exponential phase in either tryptose minimal medium or chemically defined medium. Lipase… CONTINUE READING
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