Production of knockout rats using ENU mutagenesis and a yeast-based screening assay

  title={Production of knockout rats using ENU mutagenesis and a yeast-based screening assay},
  author={Yunhong Zan and Jill D. Haag and Kai-Shun Chen and Laurie A. Shepel and Don Wigington and Yurong Wang and Rong Hu and Christine C Lopez-Guajardo and Heidi L. Brose and Katherine I Porter and Rachel A Leonard and Andrew A. Hitt and Stacy L Schommer and Anu F Elegbede and Michael N. Gould},
  journal={Nature Biotechnology},
The rat is a widely used model in biomedical research and is often the preferred rodent model in many areas of physiological and pathobiological research. Although many genetic tools are available for the rat, methods to produce gene-disrupted knockout rats are greatly needed. In this study, we developed protocols for creating N-ethyl-N-nitrosourea (ENU)-induced germline mutations in several rat strains. F1 preweanling pups from mutagenized Sprague Dawley (SD) male rats were then screened for… CONTINUE READING


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