Production of itaconic acid using metabolically engineered Escherichia coli.

  title={Production of itaconic acid using metabolically engineered Escherichia coli.},
  author={Shusuke Okamoto and Taejun Chin and Ken Hiratsuka and Yuji Aso and Yasutomo Tanaka and Tetsuya Takahashi and Hitomi Ohara},
  journal={The Journal of general and applied microbiology},
  volume={60 5},
An Escherichia coli system was engineered for the heterologous production of itaconic acid via the expression of cis-aconitate decarboxylase gene (cad), and then maximal itaconic acid levels produced by engineered E. coli were evaluated. Expression of cad in E. coli grown in Luria-Bertani (LB) medium without glucose in a test tube resulted in 0.07 g/L itaconic acid production after 78 h at 20°C. To increase itaconic acid production, E. coli recombinants were constructed by inactivating the… CONTINUE READING
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