Production of hybridoma growth factor by human monocytes.

@article{Aarden1987ProductionOH,
  title={Production of hybridoma growth factor by human monocytes.},
  author={Lucien A. Aarden and Els R de Groot and O L Schaap and Peter M. Lansdorp},
  journal={European journal of immunology},
  year={1987},
  volume={17 10},
  pages={
          1411-6
        }
}
Human mononuclear leukocytes produce a growth factor (HGF) for hybridoma and plasmacytoma cells. HGF has recently been proven to be identical to IFN-beta 2, 26-kDa protein and BSF-2. HGF can be quantitated in a proliferation assay with the HGF-dependent hybridoma cell line B13.29. By selection of an extremely sensitive variant of this cell line, we were able to measure HGF production of single cells. Limiting dilution analysis of the producing cells in combination with size, density and… CONTINUE READING
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