Production of human metabolites of cyclosporin A, AM1, AM 4 N and AM 9, by microbial conversion.

@article{Ohta2005ProductionOH,
  title={Production of human metabolites of cyclosporin A, AM1, AM 4 N and AM 9, by microbial conversion.},
  author={Kazuo Ohta and Hitosi Agematu and Tomoya Yamada and Katsura Kaneko and Toshio Tsuchida},
  journal={Journal of bioscience and bioengineering},
  year={2005},
  volume={99 4},
  pages={390-5}
}
In humans, cyclosporin A (CyA) is primarily metabolized to two hydroxylated (AM1 and AM 9) and one N-demethylated (AM 4 N) derivative. To produce these derivatives, 1237 actinomycetes were screened for their ability to convert CyA. Among them, 89 strains (7.2%) produced these derivatives from CyA. Finally, Dactylosporangium variesporum IFO 14104, Actinoplanes sp. ATCC 53771 and Streptosporangium sp. AF 935 were selected for the production of AM1, AM 4 N, and AM 9, respectively. Composition of… CONTINUE READING