Production of human compatible high mannose-type (Man5GlcNAc2) sugar chains in Saccharomyces cerevisiae.

Abstract

A yeast mutant capable of producing Man5GlcNAc2 human compatible sugar chains on glycoproteins was constructed. An expression vector for alpha-1,2-mannosidase with the "HDEL" endoplasmic reticulum retention/retrieval tag was designed and expressed in Saccharomyces cerevisiae. An in vitro alpha-1,2-mannosidase assay and Western blot analysis showed that it… (More)

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@article{Chiba1998ProductionOH, title={Production of human compatible high mannose-type (Man5GlcNAc2) sugar chains in Saccharomyces cerevisiae.}, author={Y. Chiba and M. Suzuki and S. Yoshida and Atsushi Yoshida and Hidenori Ikenaga and M. Takeuchi and Y. Jigami and Eiji Ichishima}, journal={The Journal of biological chemistry}, year={1998}, volume={273 41}, pages={26298-304} }