• Medicine
  • Published in Xenotransplantation 2007

Production of homozygous alpha-1,3-galactosyltransferase knockout pigs by breeding and somatic cell nuclear transfer.

@article{Nottle2007ProductionOH,
  title={Production of homozygous alpha-1,3-galactosyltransferase knockout pigs by breeding and somatic cell nuclear transfer.},
  author={Mark Brenton Nottle and Luke F S Beebe and Sharon J. Harrison and Stephen M. McIlfatrick and Rodney J Ashman and P. J. O'connell and Evelyn J Salvaris and Nella Fisicaro and Sandra Pommey and Peter J Cowan and Anthony J.F. d'Apice},
  journal={Xenotransplantation},
  year={2007},
  volume={14 4},
  pages={
          339-44
        }
}
We report here our experience regarding the production of double or homozygous Gal knockout (Gal KO) pigs by breeding and somatic cell nuclear transfer (SCNT). Large White x Landrace female heterozygous Gal KO founders produced using SCNT were mated with Hampshire or Duroc males to produce a F1 generation. F1 heterozygous pigs were then bred to half-sibs to produce a F2 generation which contained Gal KO pigs. To determine the viability of mating Gal KO pigs with each other, one female F2 Gal KO… CONTINUE READING

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